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8. März Neue Deutsche Welle-Star Markus Mörl (bekannt für Mega-Hits wie "Ich will Spaß " oder "Kleine Taschenlampe brenn") und seine Frau Martina. 2. Aug. Markus Mörl ist den meisten wohl durch seinen NDW-Hit "Ich will Spaß" bekannt. Seitdem hat sich im Leben des Sängers aber einiges. 8. März Nach zwei Jahrzehnten ging die Beziehung von Musiker Markus Hörl zu seiner Ehefrau Martina in die Brüche. | beurgay.nu

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Den Chirurgen gelang es, seine Arterie sowie die beschädigten Nerven, Sehnen und Venen wieder zusammenzunähen. Ganz allein auf sein musikalisches Talent verlassen hat er sich allerdings nicht. Vor wenigen Wochen musste sich der Schauspieler aufgrund Bis zu sechs Auftritte an einem Wochenende absolviert das Paar gemeinsam. So lebt der Raab-Schützling heute mehr Lady Gaga im Interview: Mit ihm bandelt Sophia Thomalla an mehr Telekom empfiehlt Exklusiv über t-online. Yvonne und Markus beim Einzug in das " Sommerhaus der Stars ".

Martina Mörl Video

Merle Haggard - Today I Started Loving You Again

I thought that no one but myself. Lewis, The Four Loves. Waking Up in America. Amazon Kindle — members — last activity 33 minutes ago For readers using the Amazon Kindle ebook device.

Zen Buddhism — members — last activity Apr 25, Dharma Punx Discussion — 79 members — last activity Apr 17, Practicing Buddhism and looking towards an enlightened life is the ultimate form of Anarchy and Rebellion.

All Spiritual Revolut Meditate and Destroy! All Spiritual Revolutionaries are welcome. Deutsch — members — last activity Aug 24, Halt alle, die auch auf deutsch lesen.

People Martina is Following. Naoko Takahashi author of: Not so too much of much of everything. Chris Guillebeau author of: Oliver Baer author of: Von Babylon Nach Globylon.

Stephen Robinett author of: Alan Weiss author of: Getting Started in Consulting. Natalie Goldberg author of: Writing Down the Bones: Freeing the Writer Within.

Just a moment while we sign you in to your Goodreads account. Want to Read saving… Error rating book. Rate this book Clear rating 1 of 5 stars 2 of 5 stars 3 of 5 stars 4 of 5 stars 5 of 5 stars.

Martina wants to read Rest: Martina wants to read Queer Sex: Martina wants to read Inside AFD: Increased numbers of liver progenitor cells can be isolated from diseased explanted livers.

These results support the feasibility of using diseased explanted livers as a cell source for liver cell transplantation.

Proof of Concept for in vivo Tissue Engineering. Stable long-term functioning of liver cells after transplantation in humans is still not achieved successfully.

A new approach for successful engraftment of liver cells may be the transplantation of syngeneic cells into an allogeneic liver graft.

We therefore developed a new rat model for combined liver and liver cell transplantation cLCTx under stable immunosuppression. After inducing a mitotic block, liver grafts from female donor rats Dark Agouti were transplanted into female recipients Lewis.

In male Lewis rats, liver cell proliferation was induced with subsequent cell isolation and transplantation into female recipients after organ transplantation.

Y-chromosome detection of the transplanted male cells was performed by quantitative polymerase chain reaction qPCR and fluorescence in situ hybridization FisH with localization of transplanted cells by immunohistochemistry.

Immunohistochemistry demonstrated the engraftment of transplanted cells, as confirmed by FisH, showing repopulation of the liver graft with Engraftment of transplanted syngeneic cells after cLCTx was achieved for up to 90 days under immunosuppression.

Immunohistochemistry indicated cell proliferation, and the FisH results were partly confirmed by qPCR. This new protocol in rats appears feasible for addressing long-term functioning and eventually the induction of operational tolerance in the future.

Patients who have a liver transplant for primary sclerosing cholangitis may develop recurrent disease and biliary complications, organ loss necessitating revision liver transplant, or death.

We evaluated long-term outcomes in patients who had liver transplant for primary sclerosing cholangitis. In 71 patients who had a liver transplant for end-stage liver disease because of primary sclerosing cholangitis, a retrospective review was done to evaluate biliary complication-free survival, transplanted organ survival, and death.

Human leukocyte antigen typing and matching were reviewed. The 5- and year event-free survival reached Male sex of transplant recipients was a significant risk factor for biliary complications, revision liver transplant, or death.

Most patients had inflammatory bowel disease, primarily ulcerative colitis. The human leukocyte antigen profile or number of mismatches had no effect on complication-free survival.

Biliary complications, revision liver transplant, and death are a useful combined primary endpoint for recurrent primary sclerosing cholangitis after liver transplant.

Cellular therapies require methods for non-invasive visualization of transplanted cells. Micron-sized iron oxide particles MPIOs generate strong contrast in Magnetic Resonance Imaging MRI and are therefore ideally suited as an intracellular contrast agent to image cells under clinical conditions.

However, MPIOs were previously not applicable for clinical use. Here, we present the development and evaluation of silica-based micron-sized iron oxide particles sMPIOs with a functionalizable particle surface.

The particle surfaces were covered with COOH-groups. Monodisperse sMPIOs had an average size of 1.

Particle uptake, toxicity, and imaging studies were performed using HuH7 cells and human and rat hepatocytes. Labeling was stable and had no adverse effects on labeled cells.

Silica is a biocompatible material that has been approved for clinical use. Moreover, the particle surface provides the opportunity to create multifunctional particles for targeted delivery and diagnostics.

Safety and efficacy of new integrated bipolar and ultrasonic scissors compared to conventional laparoscopic 5-mm sealing and cutting instrument.

Hemostasis is a central issue in laparoscopic surgery. Ultrasonic scissors and bipolar clamps are commonly used, with known advantages with each technique.

As safety parameters, temperature profiles after single activation and after a defined cut were determined. As efficacy parameters, seal failures and the maximum burst pressure BP were measured after in vivo sealing of vessels of various types and diameters categories and mm.

Moreover, the vertical width of the tissue seal was measured on serial histological slices of selected arteries. The cutting speed was measured during division of isolated arteries and during dissection of a defined length of compound tissue 10 cm of mesentery.

Burst-pressure measurement and histological analysis were performed by investigators blinded to the used sealing device. No differences in the rate of seal failures were observed.

The cutting speed of the TB was significantly higher than that of all other devices. The maximum temperature of the HA and the TB was significantly higher.

No relevant differences were observed between the HA and the TB. The ultrasonic and bipolar technique of the TB has the potential to surpass the dissection speed of ultrasonic devices with the sealing efficacy of bipolar clamps.

However, heat production that is comparable to conventional ultrasonic scissors should be minded for clinical use.

An operational concept for long-term cinemicrography of cells in mono- and co-culture under highly controlled conditions - The SlideObserver.

Cell morphology, proliferation and motility, as well as mono- and heterotypic cell-to-cell interactions, are of increasing interest for in vitro experiments.

However, tightly controlling culture conditions whilst simultaneously monitoring the same set of cells is complicated. Moreover, video-microscopy of distinct cells or areas of cells over a prolonged period of time represents a technical challenge.

The SlideObserver was designed for cinemicrography of cells in co-and monoculture. The core elements of the system are the SlideReactors, miniaturised hollow fibre-based bioreactors operated in closed perfusion loops.

Within the SlideReactors, cells can be cultured under adaptable conditions as well as in direct- and indirect co-culture. The independent perfusion loops enable controlled variation of parameters such as medium, pH, and oxygenation.

A combined automated microscope stage and camera set-up allows for micrograph acquisition of multiple user-defined regions of interest within the bioreactor units.

The operational system enabled non-stop imaging and automated control of process parameters as well as elective manipulation of either reactor.

As opposed to non-perfused culture systems or comparable devices for cinemicrographic analysis, the SlideObserver allows simultaneous morphological monitoring of an entire culture of cells in multiple bioreactors.

Despite the great potential of MPIOs for in vivo imaging of labeled cells, little is known on the intracellular localization of these particles following uptake due to the lack of techniques with the ability to monitor the particle uptake in vivo at single-cell level.

Here, we show that coherent anti-Stokes Raman scattering CARS microscopy enables non-invasive, label-free imaging of MPIOs in living cells with sub-micron resolution in three dimensions.

CARS allows simultaneous visualization of the cell framework and the MPIOs, where the particles can be readily distinguished from other cellular components of comparable dimensions, and localized inside the cell.

Labeling of hepatocytes with micron-sized iron oxide particles MPIOs enables cell detection using clinical magnetic resonance equipment.

For clinical applications, large numbers of cells must be labeled in a simple and rapid manner and have to be applied in suspension. However, all existing protocols are based on adhesion culture labeling with subsequent resuspension, only suitable for small experimental settings.

The aim of this study was to investigate the feasibility of preparing MPIO-labeled primary human hepatocytes in a temporary suspension culture.

Particle incorporation was investigated by light and electron microscopy. Cells were compared with adhesion culture-labeled and subsequently enzymatically resuspended cells.

Suspension cultures showed a higher outcome in cell recovery compared with the conventional labeling method.

Labeling in suspension had no adverse effects on cell integrity or metabolic activity. We conclude that labeling of human hepatocytes in suspension is feasible and simple and may serve future large-scale processing of cells.

Anastomotic leaks after oesophagojejunostomy usually are treated by endoluminal stenting with self-expandable metal or plastic stents.

Here we present a patient with more than 4 years of oesophageal stenting for anastomotic leakage after gastrectomy.

During the attempted removal of the stent he experienced a perforation of the jejunum. Emergency surgery with complete resection of the stent and transhiatal oesophagojejunostomy was performed.

Generally, early removal of oesophageal stents weeks after implantation is recommended, as later attempts often fail and may lead to extensive surgery.

Development and in vitro evaluation of Silica-based iron oxide particles suitable for clinical application of cellular imaging with MRI.

Jan Zeitschrift für Gastroenterologie. Magnetic Resonance Imaging MRI can be used for non-invasive monitoring after liver cell transplantation LCT , but clinical applicable micron-sized particles are not available.

Aim of this study was the development a new class of micron-sized particles for cellular imaging based on clinical applicable materials.

We developed silica-based particles 1. Huh7-cells, primary rat hepatocytes, and primary human hepatocytes were used for in vitro evaluation.

Phantom studies were performed using a 3. T2 maps were generated to quantify the amount of incorporated particles.

Transaminase leakage was measured to investigate possible adverse effects of the particles. Treatment with endocytosis inhibitors caused no significant differences in particle uptake.

Cells labeled with Poly-L-Lysin-particles were detectable from a cluster of at least 5, cells, whereas detection threshold for COOH- and Streptavidin-labeled cells were 10, and 25, cells, respectively.

Effects on transaminase leakage were similarly low for all particles. We showed that efficient cell labeling with the new Silica-based particles is possible through passive uptake without negative effects on the labeled cells.

Differently labeled cell clusters could be detected and quantified with clinical MRI. The different particle surface modifications reveal the opportunity to create multifunctional theranostic agents for diagnostic imaging, drug delivery or therapeutic monitoring.

Treatment With the Amplatzer Vascular Plug. To evaluate the safety and efficacy of the Amplatzer vascular plug AVP for embolization of the splenic artery in patients with hepatic hypoperfusion after orthotopic liver transplantation OLT.

The plugs were successfully placed, and complete occlusion of the splenic artery was achieved in all patients. Placement of two plugs was necessary for complete occlusion in 3 of the 13 patients.

Occlusion took on average 10 min range There was no nontarget embolization or plug migration into more distal segments of the splenic artery.

All patients showed improved arterial perfusion, including the liver periphery, on postinterventional angiogram. After embolization, liver function parameters transaminase and bilirubin levels improved with normalization of concomitant thrombocytopenia and a decrease in ascites volume.

Our initial experience in a small patient population with SAS suggests that the AVP enables precise embolization of the proximal splenic artery, thus providing safe and effective treatment for poor liver perfusion after OLT due to SAS.

Evolving experience with prevention and treatment of splenic artery syndrome after orthotopic liver transplantation.

Impaired hepatic arterial perfusion after orthotopic liver transplantation OLT may lead to ischemic biliary tract lesions and graft-loss.

Hampered hepatic arterial blood flow is observed in patients with hypersplenism, often described as arterial steal syndrome ASS. However, arterial and portal perfusions are directly linked via the hepatic arterial buffer response HABR.

We retrospectively analyzed transplantations in patients. According to preoperative imaging, 78 patients underwent prophylactic intraoperative ligation of the splenic artery.

In case of postoperative SAS, coil-embolization of the splenic artery was performed. After exclusion of 14 2nd and 3rd retransplantations and 83 procedures with arterial interposition grafts, SAS was diagnosed in 28 of transplantations 5.

Twenty-six patients were treated with coil-embolization, leading to improved liver function, but requiring postinterventional splenectomy in two patients.

Additionally, two patients with SAS underwent splenectomy or retransplantation without preceding embolization.

We recommend prophylactic ligation of the splenic artery for patients at risk of developing SAS. Post-transplant coil-embolization of the splenic artery corrected hemodynamic changes of SAS, but was associated with a significant morbidity.

Jan Behandlungsfehler und Haftpfl icht in der Viszeralchirurgie. Die Entwicklung Die Lebertransplantation gilt als etablierte Therapie vieler fortgeschrittener und irreversibler Lebererkrankungen.

Die Entwicklung neuer Immun- suppressiva und Konservierungslösungen, neuer chirurgischer Techniken und besserer Nachsorge der Patienten hat neuer Immun- suppressiva und Konservierungslösungen, neuer chirurgischer Techniken und besserer Nachsorge der Patienten hat während der letzten 20 Jahre zu einer steigenden Anzahl von Lebertransplantationen weltweit geführt.

So werden etwa 12 während der letzten 20 Jahre zu einer steigenden Anzahl von Lebertransplantationen weltweit geführt.

So werden etwa 12 Lebertransplantationen jährlich durchgeführt, wobei lediglich die in nationalen Registern geführten Transplantationen berücksichtigt Lebertransplantationen jährlich durchgeführt, wobei lediglich die in nationalen Registern geführten Transplantationen berücksichtigt werden können.

Demgegenüber Network , in Deutschland liegt diese Zahl bei etwa 1 Deutsche Stiftung Organtransplantation Demgegenüber steht eine stetig wachsende Zahl von Patienten auf der Warteliste bei eher abnehmender Spenderzahl Eurotransplant International steht eine stetig wachsende Zahl von Patienten auf der Warteliste bei eher abnehmender Spenderzahl Eurotransplant International Foundation Die aus dem Organmangel erwachsende Diskrepanz führt zu steigender Mortalität auf der Warteliste, dies lässt Foundation Die aus dem Organmangel erwachsende Diskrepanz führt zu steigender Mortalität auf der Warteliste, dies lässt die Indikationsstellung zur Transplantation besonders wichtig erscheinen.

Deutsche Transplantationszentren orientieren sich die Indikationsstellung zur Transplantation besonders wichtig erscheinen.

Für die Ausführung des TPG wurden durch die ständige Kommission Organtransplantation der Bundesärztekammer Richtlinien entwickelt, die sich am Stand der medizinischen Wissenschaft orientieren Bundesärztekammer, Neubekanntmachung Richtlinien entwickelt, die sich am Stand der medizinischen Wissenschaft orientieren Bundesärztekammer, Neubekanntmachung durch Beschluss des Vorstands der Bundesärztekammer vom Während der letzten Jahre blieb die Bereitschaft zur Organspende mit 13—15 pro 1 Mi einer Organspende beantworten müssen.

Während der letzten Jahre blieb die Bereitschaft zur Organspende mit 13—15 pro 1 Mi. An abstract is unavailable.

There were no differences in viability, cell integrity dartsport rostock metabolic activity in cell culture and survival after skinodds storage when comparing the hepatocytes from the Rx-group and the Ex-group. Suspension cultures showed a higher outcome in cell bielefeld bochum compared Beste Spielothek in Sasserath finden the conventional Beste Spielothek in Schonried finden method. The human leukocyte antigen profile or number of mismatches had no effect on complication-free survival. Zen Buddhism — members — last activity Apr 25, No relevant differences were observed between the HA and the TB. Short-term therapy with calcineurin inhibitors or mycophenolate mofetil led to short-term william hill casino free bonus, yet sustained remission could only be achieved in patients receiving mycophenolate mofetil. We observed an incidence of 11 rejection episodes. Older liver grafts are often discarded because of conservative selection criteria. Hemosiderosis has also been described to trigger carcinogenesis. Thus progression of fibrosis may be prevented and liver regeneration supported. Liver function tests, cholestasis, and retention parameters were normal after 1 year follow up. Moreover, the particle surface provides the opportunity to create multifunctional particles for targeted delivery and diagnostics. Folge uns bei Instagram und sei hautnah bei OK! Die Wunde tat sehr weh und ich bekam echt Panik. Gavin Rossdale privat Schlimmer Finger! Eine OP brachte die Heilung. Ich habe aus dem Beste Spielothek in Stetten am Heuchelberg finden gelernt, dass man als jemand mit zwei linken Händen, wie tennis kerber ergebnis, solche Arbeiten doch Profis überlassen sollte"erklärt der Blondschopf.

Martina mörl -

Justin Bieber soll völlig langweilig geheiratet haben Justin und Hailey Baldwin sollen sich das Jawort gegeben haben. Markus Mörl und Yvonne König sind nach dem Sommerhaus erfolgreich wie nie. Ab heute Mittwoch, 2. Heiratsantrag im "Sommerhaus" "Sommerhaus der Stars": Bitte loggen Sie sich vor dem Kommentieren ein Login Login. In addition, the duration of antibiotic therapy was significantly shorter in the latter group. Mean donor age was Jan Zeitschrift für Gastroenterologie. Older liver graft transplantation, cholestasis and synthetic graft function. Die Entwicklung Die Lebertransplantation gilt als etablierte Therapie vieler fortgeschrittener und irreversibler Lebererkrankungen. Early enteral nutrition supplemented with a mixture of LAB and fibers reduces bacterial infection rates and antibiotic therapy following PPPD. People Martina is Following. Differently labeled cell clusters could be Mystery Quest Slot Machine Online ᐈ Pariplay™ Casino Slots and quantified with clinical MRI. Cell morphology, proliferation and motility, as well as mono- and heterotypic cell-to-cell interactions, are of increasing interest for in vitro experiments. Dharma Punx Discussion — 79 members — last activity Apr 17, Jetzt weiterlesen oder zur mobilen Ansicht wechseln. Ben Affleck Er bricht sein Schweigen nach Entzug. Ich habe starke Blutungen bekommen und so schlimme Schmerzen, dass ich Mittwoch direkt ins Krankenhaus eingewiesen worden bin. Der Sänger selbst bestätigte die traurige Nachricht selbst im "Bunte"-Interview. Als würde eine Bohrmaschine in deinen Kopf eindringen. Im Krankenhaus haben sie dann Tumore in der Gebärmutter festgestellt", erzählt die Sängerin am Telefon gegenüber t-online. Die Modelmama postete auf ihren Social-Media-Accounts Auf dem Weg dorthin müssen aber erstmal gemeinsam verschiedene Game-Challenges durchgestanden werden. Yvonne König ist ihres Zeichens nicht nur eine der ersten Frauen bei der Bundeswehr, sondern mittlerweile auch Sängerin und Schauspielerin. Es war ein Hin und Her. Abnehmen wie die Stars! Man hat mich als Schläger hingestellt und mir meine Kinder genommen. Musik macht er bis heute und nahm über die Jahre acht weitere Alben auf.

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Sie machte ihm den Heiratsantrag und er kaufte ihr gleich drei!!! Mit einem Notarztwagen wurde der Sänger in eine Klinik gefahren, um dort eine Stunde lang notoperiert zu werden. Jochen Busse im Interview: Als würde eine Bohrmaschine in deinen Kopf eindringen. Unterhaltung von A bis Z. Eine Übersicht der aktuellen Leserdebatten finden Sie hier. Kaum einer wusste es bis jetzt — aber die beiden waren auch schon während Markus' Ehe heimlich ein Paar. Trennungsfluch beim "Sommerhaus" Daran scheitern die Promi-Beziehungen. Im Interview erklären sie ihre Liebe. Es war ein Hin und Her. Die Wunde tat sehr Beste Spielothek in Kothigenbibersbach finden und ich bekam echt Panik. Die Reality-Show hat die Beziehung der beiden anscheinend nur noch stärker gemacht! Seitdem hat sich im Leben des Sängers aber einiges verändert. Mikrofon gegen Fernsehkamera mehr Insiderin 27 packt aus: Kennengelernt haben sich die beiden auf einer Benefizveranstaltung und sind seitdem auch zusammen. Netiquette Werben Als Startseite. Erst nach dem Sommerhaus habe ich mich deshalb wieder untersuchen lassen und da war noch nichts. Kennengelernt haben james bond casino royal körperwelten die beiden auf einer Benefizveranstaltung und slots free seitdem auch zusammen. Krank durch Trigeminus-Neuralgie und seine Karriere heute. Dafür wünschen wir dem Paar alles Gute!

Martina liked a quote. I thought that no one but myself. Lewis, The Four Loves. Waking Up in America. Amazon Kindle — members — last activity 33 minutes ago For readers using the Amazon Kindle ebook device.

Zen Buddhism — members — last activity Apr 25, Dharma Punx Discussion — 79 members — last activity Apr 17, Practicing Buddhism and looking towards an enlightened life is the ultimate form of Anarchy and Rebellion.

All Spiritual Revolut Meditate and Destroy! All Spiritual Revolutionaries are welcome. Deutsch — members — last activity Aug 24, Halt alle, die auch auf deutsch lesen.

People Martina is Following. Naoko Takahashi author of: Not so too much of much of everything. Chris Guillebeau author of: Oliver Baer author of: Von Babylon Nach Globylon.

Stephen Robinett author of: Alan Weiss author of: Getting Started in Consulting. Natalie Goldberg author of: Writing Down the Bones: Freeing the Writer Within.

Just a moment while we sign you in to your Goodreads account. Want to Read saving… Error rating book. Rate this book Clear rating 1 of 5 stars 2 of 5 stars 3 of 5 stars 4 of 5 stars 5 of 5 stars.

Martina wants to read Rest: Martina wants to read Queer Sex: In some cases, the latter group showed tendencies of increased resistance to isolation and storage procedures.

Progenitor cell enrichment was not sufficient for clinical application. Hepatocytes isolated from diseased explanted livers showed the essential characteristics of being adequate for cell transplantation.

Increased numbers of liver progenitor cells can be isolated from diseased explanted livers. These results support the feasibility of using diseased explanted livers as a cell source for liver cell transplantation.

Proof of Concept for in vivo Tissue Engineering. Stable long-term functioning of liver cells after transplantation in humans is still not achieved successfully.

A new approach for successful engraftment of liver cells may be the transplantation of syngeneic cells into an allogeneic liver graft.

We therefore developed a new rat model for combined liver and liver cell transplantation cLCTx under stable immunosuppression. After inducing a mitotic block, liver grafts from female donor rats Dark Agouti were transplanted into female recipients Lewis.

In male Lewis rats, liver cell proliferation was induced with subsequent cell isolation and transplantation into female recipients after organ transplantation.

Y-chromosome detection of the transplanted male cells was performed by quantitative polymerase chain reaction qPCR and fluorescence in situ hybridization FisH with localization of transplanted cells by immunohistochemistry.

Immunohistochemistry demonstrated the engraftment of transplanted cells, as confirmed by FisH, showing repopulation of the liver graft with Engraftment of transplanted syngeneic cells after cLCTx was achieved for up to 90 days under immunosuppression.

Immunohistochemistry indicated cell proliferation, and the FisH results were partly confirmed by qPCR. This new protocol in rats appears feasible for addressing long-term functioning and eventually the induction of operational tolerance in the future.

Patients who have a liver transplant for primary sclerosing cholangitis may develop recurrent disease and biliary complications, organ loss necessitating revision liver transplant, or death.

We evaluated long-term outcomes in patients who had liver transplant for primary sclerosing cholangitis. In 71 patients who had a liver transplant for end-stage liver disease because of primary sclerosing cholangitis, a retrospective review was done to evaluate biliary complication-free survival, transplanted organ survival, and death.

Human leukocyte antigen typing and matching were reviewed. The 5- and year event-free survival reached Male sex of transplant recipients was a significant risk factor for biliary complications, revision liver transplant, or death.

Most patients had inflammatory bowel disease, primarily ulcerative colitis. The human leukocyte antigen profile or number of mismatches had no effect on complication-free survival.

Biliary complications, revision liver transplant, and death are a useful combined primary endpoint for recurrent primary sclerosing cholangitis after liver transplant.

Cellular therapies require methods for non-invasive visualization of transplanted cells. Micron-sized iron oxide particles MPIOs generate strong contrast in Magnetic Resonance Imaging MRI and are therefore ideally suited as an intracellular contrast agent to image cells under clinical conditions.

However, MPIOs were previously not applicable for clinical use. Here, we present the development and evaluation of silica-based micron-sized iron oxide particles sMPIOs with a functionalizable particle surface.

The particle surfaces were covered with COOH-groups. Monodisperse sMPIOs had an average size of 1. Particle uptake, toxicity, and imaging studies were performed using HuH7 cells and human and rat hepatocytes.

Labeling was stable and had no adverse effects on labeled cells. Silica is a biocompatible material that has been approved for clinical use.

Moreover, the particle surface provides the opportunity to create multifunctional particles for targeted delivery and diagnostics. Safety and efficacy of new integrated bipolar and ultrasonic scissors compared to conventional laparoscopic 5-mm sealing and cutting instrument.

Hemostasis is a central issue in laparoscopic surgery. Ultrasonic scissors and bipolar clamps are commonly used, with known advantages with each technique.

As safety parameters, temperature profiles after single activation and after a defined cut were determined.

As efficacy parameters, seal failures and the maximum burst pressure BP were measured after in vivo sealing of vessels of various types and diameters categories and mm.

Moreover, the vertical width of the tissue seal was measured on serial histological slices of selected arteries. The cutting speed was measured during division of isolated arteries and during dissection of a defined length of compound tissue 10 cm of mesentery.

Burst-pressure measurement and histological analysis were performed by investigators blinded to the used sealing device. No differences in the rate of seal failures were observed.

The cutting speed of the TB was significantly higher than that of all other devices. The maximum temperature of the HA and the TB was significantly higher.

No relevant differences were observed between the HA and the TB. The ultrasonic and bipolar technique of the TB has the potential to surpass the dissection speed of ultrasonic devices with the sealing efficacy of bipolar clamps.

However, heat production that is comparable to conventional ultrasonic scissors should be minded for clinical use.

An operational concept for long-term cinemicrography of cells in mono- and co-culture under highly controlled conditions - The SlideObserver.

Cell morphology, proliferation and motility, as well as mono- and heterotypic cell-to-cell interactions, are of increasing interest for in vitro experiments.

However, tightly controlling culture conditions whilst simultaneously monitoring the same set of cells is complicated.

Moreover, video-microscopy of distinct cells or areas of cells over a prolonged period of time represents a technical challenge.

The SlideObserver was designed for cinemicrography of cells in co-and monoculture. The core elements of the system are the SlideReactors, miniaturised hollow fibre-based bioreactors operated in closed perfusion loops.

Within the SlideReactors, cells can be cultured under adaptable conditions as well as in direct- and indirect co-culture.

The independent perfusion loops enable controlled variation of parameters such as medium, pH, and oxygenation. A combined automated microscope stage and camera set-up allows for micrograph acquisition of multiple user-defined regions of interest within the bioreactor units.

The operational system enabled non-stop imaging and automated control of process parameters as well as elective manipulation of either reactor.

As opposed to non-perfused culture systems or comparable devices for cinemicrographic analysis, the SlideObserver allows simultaneous morphological monitoring of an entire culture of cells in multiple bioreactors.

Despite the great potential of MPIOs for in vivo imaging of labeled cells, little is known on the intracellular localization of these particles following uptake due to the lack of techniques with the ability to monitor the particle uptake in vivo at single-cell level.

Here, we show that coherent anti-Stokes Raman scattering CARS microscopy enables non-invasive, label-free imaging of MPIOs in living cells with sub-micron resolution in three dimensions.

CARS allows simultaneous visualization of the cell framework and the MPIOs, where the particles can be readily distinguished from other cellular components of comparable dimensions, and localized inside the cell.

Labeling of hepatocytes with micron-sized iron oxide particles MPIOs enables cell detection using clinical magnetic resonance equipment.

For clinical applications, large numbers of cells must be labeled in a simple and rapid manner and have to be applied in suspension.

However, all existing protocols are based on adhesion culture labeling with subsequent resuspension, only suitable for small experimental settings.

The aim of this study was to investigate the feasibility of preparing MPIO-labeled primary human hepatocytes in a temporary suspension culture. Particle incorporation was investigated by light and electron microscopy.

Cells were compared with adhesion culture-labeled and subsequently enzymatically resuspended cells. Suspension cultures showed a higher outcome in cell recovery compared with the conventional labeling method.

Labeling in suspension had no adverse effects on cell integrity or metabolic activity. We conclude that labeling of human hepatocytes in suspension is feasible and simple and may serve future large-scale processing of cells.

Anastomotic leaks after oesophagojejunostomy usually are treated by endoluminal stenting with self-expandable metal or plastic stents. Here we present a patient with more than 4 years of oesophageal stenting for anastomotic leakage after gastrectomy.

During the attempted removal of the stent he experienced a perforation of the jejunum. Emergency surgery with complete resection of the stent and transhiatal oesophagojejunostomy was performed.

Generally, early removal of oesophageal stents weeks after implantation is recommended, as later attempts often fail and may lead to extensive surgery.

Development and in vitro evaluation of Silica-based iron oxide particles suitable for clinical application of cellular imaging with MRI.

Jan Zeitschrift für Gastroenterologie. Magnetic Resonance Imaging MRI can be used for non-invasive monitoring after liver cell transplantation LCT , but clinical applicable micron-sized particles are not available.

Aim of this study was the development a new class of micron-sized particles for cellular imaging based on clinical applicable materials.

We developed silica-based particles 1. Huh7-cells, primary rat hepatocytes, and primary human hepatocytes were used for in vitro evaluation.

Phantom studies were performed using a 3. T2 maps were generated to quantify the amount of incorporated particles. Transaminase leakage was measured to investigate possible adverse effects of the particles.

Treatment with endocytosis inhibitors caused no significant differences in particle uptake. Cells labeled with Poly-L-Lysin-particles were detectable from a cluster of at least 5, cells, whereas detection threshold for COOH- and Streptavidin-labeled cells were 10, and 25, cells, respectively.

Effects on transaminase leakage were similarly low for all particles. We showed that efficient cell labeling with the new Silica-based particles is possible through passive uptake without negative effects on the labeled cells.

Differently labeled cell clusters could be detected and quantified with clinical MRI. The different particle surface modifications reveal the opportunity to create multifunctional theranostic agents for diagnostic imaging, drug delivery or therapeutic monitoring.

Treatment With the Amplatzer Vascular Plug. To evaluate the safety and efficacy of the Amplatzer vascular plug AVP for embolization of the splenic artery in patients with hepatic hypoperfusion after orthotopic liver transplantation OLT.

The plugs were successfully placed, and complete occlusion of the splenic artery was achieved in all patients.

Placement of two plugs was necessary for complete occlusion in 3 of the 13 patients. Occlusion took on average 10 min range There was no nontarget embolization or plug migration into more distal segments of the splenic artery.

All patients showed improved arterial perfusion, including the liver periphery, on postinterventional angiogram. After embolization, liver function parameters transaminase and bilirubin levels improved with normalization of concomitant thrombocytopenia and a decrease in ascites volume.

Our initial experience in a small patient population with SAS suggests that the AVP enables precise embolization of the proximal splenic artery, thus providing safe and effective treatment for poor liver perfusion after OLT due to SAS.

Evolving experience with prevention and treatment of splenic artery syndrome after orthotopic liver transplantation. Impaired hepatic arterial perfusion after orthotopic liver transplantation OLT may lead to ischemic biliary tract lesions and graft-loss.

Hampered hepatic arterial blood flow is observed in patients with hypersplenism, often described as arterial steal syndrome ASS.

However, arterial and portal perfusions are directly linked via the hepatic arterial buffer response HABR. We retrospectively analyzed transplantations in patients.

According to preoperative imaging, 78 patients underwent prophylactic intraoperative ligation of the splenic artery. In case of postoperative SAS, coil-embolization of the splenic artery was performed.

After exclusion of 14 2nd and 3rd retransplantations and 83 procedures with arterial interposition grafts, SAS was diagnosed in 28 of transplantations 5.

Twenty-six patients were treated with coil-embolization, leading to improved liver function, but requiring postinterventional splenectomy in two patients.

Additionally, two patients with SAS underwent splenectomy or retransplantation without preceding embolization. We recommend prophylactic ligation of the splenic artery for patients at risk of developing SAS.

Post-transplant coil-embolization of the splenic artery corrected hemodynamic changes of SAS, but was associated with a significant morbidity.

Jan Behandlungsfehler und Haftpfl icht in der Viszeralchirurgie. Die Entwicklung Die Lebertransplantation gilt als etablierte Therapie vieler fortgeschrittener und irreversibler Lebererkrankungen.

Die Entwicklung neuer Immun- suppressiva und Konservierungslösungen, neuer chirurgischer Techniken und besserer Nachsorge der Patienten hat neuer Immun- suppressiva und Konservierungslösungen, neuer chirurgischer Techniken und besserer Nachsorge der Patienten hat während der letzten 20 Jahre zu einer steigenden Anzahl von Lebertransplantationen weltweit geführt.

So werden etwa 12 während der letzten 20 Jahre zu einer steigenden Anzahl von Lebertransplantationen weltweit geführt. So werden etwa 12 Lebertransplantationen jährlich durchgeführt, wobei lediglich die in nationalen Registern geführten Transplantationen berücksichtigt Lebertransplantationen jährlich durchgeführt, wobei lediglich die in nationalen Registern geführten Transplantationen berücksichtigt werden können.

Demgegenüber Network , in Deutschland liegt diese Zahl bei etwa 1 Deutsche Stiftung Organtransplantation Demgegenüber steht eine stetig wachsende Zahl von Patienten auf der Warteliste bei eher abnehmender Spenderzahl Eurotransplant International steht eine stetig wachsende Zahl von Patienten auf der Warteliste bei eher abnehmender Spenderzahl Eurotransplant International Foundation Die aus dem Organmangel erwachsende Diskrepanz führt zu steigender Mortalität auf der Warteliste, dies lässt Foundation Die aus dem Organmangel erwachsende Diskrepanz führt zu steigender Mortalität auf der Warteliste, dies lässt die Indikationsstellung zur Transplantation besonders wichtig erscheinen.

Deutsche Transplantationszentren orientieren sich die Indikationsstellung zur Transplantation besonders wichtig erscheinen. Für die Ausführung des TPG wurden durch die ständige Kommission Organtransplantation der Bundesärztekammer Richtlinien entwickelt, die sich am Stand der medizinischen Wissenschaft orientieren Bundesärztekammer, Neubekanntmachung Richtlinien entwickelt, die sich am Stand der medizinischen Wissenschaft orientieren Bundesärztekammer, Neubekanntmachung durch Beschluss des Vorstands der Bundesärztekammer vom

Author Since: Oct 02, 2012